· Simultaneous optical and 8
channels of electrical recording
· Software control of a shutter and a stimulator
· 3 available modes for triggering of acquisition/averaging:
· Analogue output for voltage commands
The acquisition interface is system
dependent. The acquisition interface for a NeuroCCD-SMQ
system is shown in the picture. A variety of options
exist for acquisition, including three major acquisition/averaging
NeuroPlex software also controls
various aspects of the experiment, such as the shutter,
the stimulator, and the camera fans (NeuroCCD).
The data acquisition software is specific
to a particular PC based analogue-to-digital converter card.
Some capabilities of the acquisition section are:
Three types of averaging are
from a trigger provided by the computer.
2. Averaging from the occurrence of
a spike on one of the recorded channels.
3. Averaging from the occurrence of
an external event such as the EKG.
In this mode, alternate trials with and without a stimulus
can be subtracted to reduce heart-beat artifacts.
For the Photodiode array system a Subset
or Binning of the diodes can be selected for acquisition.
Using a subset allows an increase in the frame rate above
1.6 kHz and/or a reduction in the size of the resulting data
file. For NeuroCCD 2x2 binning can be used to increase the
frame rate, reduce the data storage requirements, and/or reduce
the relative dark-noise contribution
The DC level of the amplifier output
can be measured directly or can be measured after subtracting
the DC level with no light.
parameters under the user's control are:
1. file name,
2. number of points (frames) per detector,
3. frame interval,
4. number of trials (for averaging),
5. interval between trials (for averaging),
6. and interval between light on and
start of sampling.