| Calcium Imaging |
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Action potentials induced Voltage-gated Ca2+ entry into a Drosophila motor nerve terminal.Data collected during the 2013 Drosophila Neurobiology: Genes, Circuits & Behavior course at CSHL.
provided by Gregory Macleod. |
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Comparison of Ca2+ signals from spine and adjacent dendrites in layers 1a and 1b in the Piriform Cortex.
Cells were filled with OGB6F 200uM and imaged via a spinning disc confocal system synchronized with a NeuroCCD.
Provided by Dietmar Schmitz and Friedrich W. Johenning, Charite?-Universitatsmedizin Berlin (J. Neuroscience, 2009) |
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 | Grey-scale display of the input to the mouse olfactory bulb in response to stimulation with two concentrations of hexanone and butanone.
At both low and high odorant concentrations the maps are different for the two odorants (see arrows). In the mouse the maps change with odorant concentration; more glomeruli are activated with increasing concentration. Provided by Matt Wachowiak and Larry Cohen, Yale University. (Neuron, 2001) |
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Pseudocolor display of the olfactory bulb response to the application of 1.7% hexonal to the olfactory epithelium in the turtle.
Provided by Matt Wachowiak, Larry Cohen, and Michal Zochowski, Yale University. (J. Neurophysiology, 2002) |
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Calcium signals from nerve terminals of receptor neurons in the mouse olfactory bulb in response to an odor presentation (2% benzaldehyde).
Provided by Dejan Vucinic, Larry Cohen and Stratos Kosmidis, Yale University |
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 | Ca transients induced by sodium action potentials.
The Calcium indicator Rhod?2 was intracellularly injected with a patch pipette. Fluorescence changes were detected using a frame rate 2.7 kHz. Provided by Hiroyoshi Miyakawa, Masashi Inoue and Yoshihisa Kudo, Laboratory of Cellular Neurobiology, Tokyo University of Pharmacy and Life Science.
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| Membrane Potential Imaging |
 | Voltage imaging of the action potential signals from terminal branches of the dendritic tuft of a rat mitral cell in an olfactory bulb slice.
Comparison between recordings at 2KHz and 10KHz frame rates.Provided by Maja Djurisic and Dejan Zecevic, Yale University. |
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 | Voltage-sensitive dye imaging of action potential backpropagation in L5 neocortical pyramidal neuron (2).
NeuroPlex ?Multiple frames? presentation of the data shown in (1) above.
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 | Voltage-sensitive dye imaging of action potential backpropagation in L5 neocortical pyramidal neuron (1).
provided by Srdjan Antic, University of Connecticut Health Center.
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 | Action potential initiation and propagation in a mitral cell in horizontal slice of the rat olfactory bulb : Two consecutive spikes evoked by a single stimulus have different initiation sites and propagation patterns.
Provided by Maja Djurisic, Srdjan Antic, Wei R. Chen, and Dejan Zecevic, Yale University. (J. Neuroscience, 2004) |
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 | Action potential in a hippocampus CA1 pyramidal neuron detected with a voltage-sensitive-dye (JPW1114). Provided by Hiroyoshi Miyakawa, Masashi Inoue and Yoshihisa Kudo, Laboratory of Cellular Neurobiology, Tokyo University of Pharmacy and Life Science. |
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| Double staining |
 | Optical Recording from a Single CA1 Pyramidal Neuron loaded with both the Voltage Sensitive Dye JPW302 and the Ca Indicator BIS-FURA-2.
Provided by Marco Canepari, Maja Djurisic and Dejan Zecevic, Yale University |
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| TIRF |
 | TIRF imaging of AtT20 cells in culture, using the dye ANNINE-6. provided by Dorte Madsen,Christian Frokjear-Jensen,Bernd Kuhn and Robert H. Chow, Keck school of medicine of USC |
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. Changes in fluorescence were measured with a NeuroCCD-SMQ camera at 1000 fps. )
 Ca Signals from a spine and dendrite in layer 1a, in response to an EPSP, 3 APs and a combination of both. B) Same for Layer 1b. )
 was used to stain the olfactory bulb.
Changes of fluorescence intensity were recorded with a NeuroCCD-SM256; resolution = 128X128 .
A. The three maps were made at the three times shown by the black arrows in section B. The maps change with time reflecting the fact that the time course of signals differ from glomerulus to glomerulus.
B. Time course of the calcium signals from three different glomeruli (pointed to by arrows of same colors). )
. Full frame (80 x 80 pixels (B)) recording at 2 KHz frame rate (D) and partial readout (80 x 12 pixels (C)) recording at 10 KHz frame rate (E). The signal is undersampled at 2 KHz because the upstroke of the spike is shorter
than 0.5 ms at 35 C.
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 Data acquisition frame rate was 2.7 kHz (370 microseconds per frame). The 15 frames shown cover the first 5.18 ms of the trace shown in B. (B) One pixel output (proximal apical dendrite) is displayed against the pseudo-colour scale used to scale pixels shown in A. Red dot marks the first sample point in the sequence of frames shown in A. )
 Somatic whole-cell recording and corresponding optical signal from soma. Image of neuron is shown below. (B, C and D) Optical signals from apical B, basal C and oblique D dendrites showing change in amplitude and timecourse with backpropagation. )
