The ratiometric method is the
most frequently used for measurements of intracellular Calcium
concentrations [Ca2+]i . This method uses the property of some
dyes (like Fura-2) to change their excitation spectrum upon
binding of Calcium ions. In that circumstance, the ratio of
the fluorescence intensities excited by 2 different wavelengths
is dependent upon [Ca2+]i. Importantly, this ratio is independent
of indicator concentration, optical path length, and illumination
intensity. Therefore, a dual-wavelength measurement is independent
of bleaching, dye leakage and small volume changes that are
frequently observed during imaging experiments. |
The Cairn Optoscan monochromator
is a high speed, high resolution wavelength changer, incorporating
nanometer precision of both center wavelength and bandwidth
settings. Wavelength and bandwidth changes are achieved in milliseconds,
with fast galvanometers driving both the grating and slit positions
to give full dynamic control of optical throughput. The Optoscan
was designed with biological fluorescence measurements in mind.
When used together with our NeuroCCD-SMQ imaging system, it
forms a powerful and fast fluorescence detection system.
Main Features:
· Millisecond control of center wavelength
· Nanometer wavelength precision over broad spectrum
· Dynamic bandwidth control
· Microsecond timing precision
· High throughput f/2 optics
· External iris diaphragm for intensity control
· Fast electronic shuttering facility
· Compact design
· Synchronized with NeuroCCD-SMQ imaging system
· Controlled from a user-friendly interface within NeuroPlex,
both on- and off-line
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